TAME, Ubiquitin Ligase APC/C Inhibitor

TAME is a competitive inhibitor of the ubiquitin ligase anaphase-promoting complex/cyclosome (APC/C). It promotes Cdc20 autoubiquitination and inhibits cyclin proteolysis in mitotic Xenopus oocyte extracts (IC50 ~ 12 μM) and blocks cyclin degradation in interphase extract.

Product information

CAS Number: 1784-03-8

Molecular Weight: 378.87

Formula: C14H23ClN4O4S

Chemical Name: (S)-methyl 5-guanidino-2-(4-methylphenylsulfonamido)pentanoate, hydrochloride

Smiles: Cl.CC1C=CC(=CC=1)S(=O)(=O)N[C@@H](CCCNC(N)=N)C(=O)OC

InChiKey: JIQFFACVQXXHMY-YDALLXLXSA-N

InChi: InChI=1S/C14H22N4O4S.ClH/c1-10-5-7-11(8-6-10)23(20,21)18-12(13(19)22-2)4-3-9-17-14(15)16;/h5-8,12,18H,3-4,9H2,1-2H3,(H4,15,16,17);1H/t12-;/m0./s1

Technical Data

Appearance: Solid Power.

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO and water up to 100 mM

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥360 days if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined.

HS Tariff Code: 382200

How to use

In Vitro:

TAME was used at 50-200 µM final concentration in various in vitro assays.

References:

1. Zeng X, et al. Pharmacologic inhibition of the anaphase-promoting complex induces a spindle checkpoint-dependent mitotic arrest in the absence of spindle damage. (2010) Cancer Cell. 18(4):382-95.
2. Zeng X, et al. An APC/C inhibitor stabilizes cyclin B1 by prematurely terminating ubiquitination. (2012) Nat Chem Biol. 8(4):383-92.

Products are for research use only. Not for human use.