详情描述
Thiazovivin is a selective, cell-permeable small molecule that inhibits ROCK activity at a concentration 2 µM. Thiazovivin improves stemness maintenance of embryo-derived stem-like cells under chemically defined culture conditions in cattle. Thiazovivin directly targets Rho-associated kinase (ROCK) and increases expression of pluripotency factors. The process using thiazovivin could be easier, faster and more cost effective than transgene integration into somatic cells.
Product information
CAS Number: 1226056-71-8
Molecular Weight: 311.36
Formula: C15H13N5OS
Chemical Name: N-Benzyl-2-(pyrimidin-4-ylamino)thiazole-4-carboxamide
Smiles: O=C(NCC1C=CC=CC=1)C1=CSC(NC2=CC=NC=N2)=N1
InChiKey: DOBKQCZBPPCLEG-UHFFFAOYSA-N
InChi: InChI=1S/C15H13N5OS/c21-14(17-8-11-4-2-1-3-5-11)12-9-22-15(19-12)20-13-6-7-16-10-18-13/h1-7,9-10H,8H2,(H,17,21)(H,16,18,19,20)
Technical Data
Appearance: Solid Power.
Purity: ≥98% (or refer to the Certificate of Analysis)
Solubility: DMSO up to 100 mM
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥360 days if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined.
HS Tariff Code: 382200
How to use
In Vitro:
Thiazovivin is usually used at 0.5-2 μM final concentration in cell culture.
References:
- Lin T, et al. A chemical platform for improved induction of human iPSCs. (2009) Nat. Methods. 6(11), 805-8.
- Xu Y, et al. Revealing a core signaling regulatory mechanism for pluripotent stem cell survival and self-renewal by 小分子化合物，新品. (2010) Proc. Natl. Acad. Sci. 107, 8129.
Products are for research use only. Not for human use.