DCVC

DCVC (S-[(1E)-1, 2-dichloroethenyl]--L-cysteine) is a bioactive metabolite of trichloroethylene (TCE). DCVC inhibits pathogen-stimulated pro-inflammatory cytokines IL-1β, IL-8, and TNF-α release from tissue cultures.

Product information

CAS Number: 13419-46-0

Molecular Weight: 216.09

Formula: C5H7Cl2NO2S

Chemical Name: (2R)-2-amino-3-{[(E)-1, 2-dichloroethenyl]sulfanyl}propanoic acid

Smiles: N[C@@H](CS/C(/Cl)=C\Cl)C(O)=O

InChiKey: PJIHCWJOTSJIPQ-PEQLYWQKSA-N

InChi: InChI=1S/C5H7Cl2NO2S/c6-1-4(7)11-2-3(8)5(9)10/h1,3H,2,8H2,(H,9,10)/b4-1-/t3-/m0/s1

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO : 2.5 mg/mL (11.57 mM; Need ultrasonic) H2O : 1.034 mg/mL (4.79 mM; Need ultrasonic and warming)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥360 days if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

Extraplacental membranes are cultured for 4, 8, and 24h with the trichloroethylene (TCE) metabolite S-(1, 2-dichlorovinyl)-l-cysteine (DCVC) in the absence or presence of lipoteichoic acid (LTA) or lipopolysaccharide (LPS) to simulate infection. In addition, membranes were cocultured with DCVC and Group B Streptococcus (GBS). DCVC (5-50μM) significantly inhibited LTA-, LPS-, and GBS-stimulated cytokine release from tissue cultures as early as 4h. DCVC inhibits pathogen-stimulated cytokine (IL-1β, IL-8, and TNF-α) release in a concentration-dependent manner.

References:

1. Boldenow E, et al. The trichloroethylene metabolite S-(1,2-dichlorovinyl)-l-cysteine but not trichloroacetate inhibits pathogen-stimulated TNF-α in human extraplacental membranes in vitro. Reprod Toxicol. 2015 Apr;52:1-6.
2. Lash LH, et al. Multigenerational study of chemically induced cytotoxicity and proliferation in cultures of human proximal tubular cells. Int J Mol Sci. 2014 Nov 18;15(11):21348-65.

Products are for research use only. Not for human use.