Ammonium iron(III) citrate

Ammonium iron(III) citrate (Ammonium ferric citrate), a physiological form of nonetransferrin-bound iron, induces intracellular iron overload to cause ferroptosis. Ammonium iron(III) citrate can enhance protein production.

Product information

CAS Number: 1185-57-5

Molecular Weight: 261.98

Formula: C6H8FeNO7

Synonym:

Ammonium ferric citrate

FAC

Chemical Name: azane;2-hydroxypropane-1, 2, 3-tricarboxylate;iron(3+)

Smiles: N.[Fe+3].[O-]C(=O)C(O)(CC([O-])=O)CC([O-])=O

InChiKey: FRHBOQMZUOWXQL-UHFFFAOYSA-K

InChi: InChI=1S/C6H8O7.Fe.H3N/c7-3(8)1-6(13,5(11)12)2-4(9)10;;/h13H,1-2H2,(H,7,8)(H,9,10)(H,11,12);;1H3/q;+3;/p-3

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: H2O : 33.33 mg/mL (Need ultrasonic) DMSO : < 1 mg/mL (ultrasonic) (insoluble or slightly soluble)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥360 days if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

Ammonium iron(III) citrate (Ammonium ferric citrate; 1, 5, 10, 15 mM; 24 hours) with 5 mM induces cell death in HT1080 cells. AML12 cells were highly resistant to Ammonium iron(III) citrate, as they maintained cell viability about 80%. Ammonium iron(III) citrate can be actively transported into cells by specific transporter. Ammonium iron(III) citrate (6, 12, 18 mg/L) has a higher biomas for Chlorella vulgaris FSP-E (CV) in BG-11 medium with 12 mg/L and 18 mg/L.

References:

1. Shenglin Fang, et al. Effects of intracellular iron overload on cell death and identification of potent cell death inhibitors. Biochem Biophys Res Commun. 2018 Sep 3;503(1):297-303.
2. Yu-Cheng Lai, et al. Towards protein production and application by using Chlorella species as circular economy. Bioresour Technol. 2019 Oct;289:121625.

Products are for research use only. Not for human use.