DMOG, Prolyl 4-hydroxylase (P4H) Inhibitor

DMOG is a selective, competitive and cell permeable prolyl 4-hydroxylase (P4H) inhibitor. It inhibits hypoxia-inducible factor α (HIF-α) prolyl hydroxylase (HIF-PH). It acts to stabilize HIF-1α expression at normal oxygen tensions in cultured cells, at concentrations between 0.1 and 1 mM. DMOG acts as a pro-angiogenic compound, acting via the HIF-1α system.

Product information

CAS Number: 89464-63-1

Molecular Weight: 175.14

Formula: C6H9NO5

Chemical Name: methyl 2-((2-methoxy-2-oxoethyl)amino)-2-oxoacetate

Smiles: COC(=O)CNC(=O)C(=O)OC

InChiKey: BNJOZDZCRHCODO-UHFFFAOYSA-N

InChi: InChI=1S/C6H9NO5/c1-11-4(8)3-7-5(9)6(10)12-2/h3H2,1-2H3,(H,7,9)

Technical Data

Appearance: Solid Power.

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO up to 100 mM; Water up to 100 mM

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥360 days if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined.

HS Tariff Code: 382200

How to use

In Vitro:

DMOG was used at 100 µM final concentration in vitro and in cellular assays.

References:

1. Jaakkola P, et al. Targeting of HIF-alpha to the von Hippel-Lindau ubiquitylation complex by O2-regulated prolyl hydroxylation. (2001) Science. 292(5516):468-72.
2. Ayrapetov MK, et al. Activation of Hif1α by the prolylhydroxylase inhibitor dimethyoxalyglycine decreases radiosensitivity. (2011) PLoS One. 6(10):e26064.
3. Barnucz E, et al. Prolyl-hydroxylase inhibition preserves endothelial cell function in a rat model of vascular ischemia reperfusion injury. (2013) J Pharmacol Exp Ther. 345(1):25-31.

Products are for research use only. Not for human use.