详情描述
Pepstatin A Janelia Fluor® 526 is a fluorogenic green-emitting lysosome probe. No wash step is required when used for multicolor microscopy experiments. Pepstatin A Janelia Fluor® 526 can be used for live cell imaging, in confocal microscopy and super-resolution microscopy techniques including 3D-SIM (structured illumination microscopy) and STED, as well as two-color lattice light-sheet microscopy (lls). Excitation maximum = 530 nm, emission maximum = 549 nm.
Product information
CAS Number: 2410614-07-0
Molecular Weight: 1442.51
Formula: C69H92F9N9O14
Synonym:
Pepstatin A JF526
Chemical Name: 2-(3-(3, 3-Difluoroazetidin-1-ium-1-ylidene)-6-(3, 3-difluoroazetidin-1-yl)-2, 7-difluoro-3H-xanthen-9-yl)-4-(((6S, 9S, 12S, 13S, 17S, 20S, 21S)-13, 21-dihydroxy-12, 20-diisobutyl-6, 9-diisopropyl-2, 17-dimethyl-4, 7, 10, 15, 18, 23-hexaoxo-5, 8, 11, 16, 19, 24-hexaazatriacontan-30-yl)carbamoyl)benzoate trifluoroacetate
Smiles: C[C@H](NC(=O)C[C@H](O)[C@H](CC(C)C)NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)CC(C)C)C(C)C)C(C)C)C(=O)N[C@@H](CC(C)C)[C@@H](O)CC(=O)NCCCCCCNC(=O)C1=CC(C2C3=CC(F)=C(C=C3OC3=CC(C(F)=CC3=2)=[N+]2CC(F)(F)C2)N2CC(F)(F)C2)=C(C=C1)C([O-])=O.OC(=O)C(F)(F)F
InChiKey: JONSFJPZCPEZFP-WKVYEHBGSA-N
InChi: InChI=1S/C67H91F6N9O12.C2HF3O2/c1-34(2)20-47(77-61(88)39(11)76-57(87)29-52(84)48(21-35(3)4)78-63(90)60(38(9)10)80-64(91)59(37(7)8)79-56(86)22-36(5)6)51(83)28-55(85)74-18-14-12-13-15-19-75-62(89)40-16-17-41(65(92)93)42(23-40)58-43-24-45(68)49(81-30-66(70,71)31-81)26-53(43)94-54-27-50(46(69)25-44(54)58)82-32-67(72,73)33-82;3-2(4,5)1(6)7/h16-17,23-27,34-39,47-48,51-52,59-60,83-84H,12-15,18-22,28-33H2,1-11H3,(H7-,74,75,76,77,78,79,80,85,86,87,88,89,90,91,92,93);(H,6,7)/t39-,47-,48-,51-,52-,59-,60-;/m0./s1
Technical Data
Appearance: Solid Power
Purity: ≥98% (or refer to the Certificate of Analysis)
Solubility: Soluble in DMSO
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined
HS Tariff Code: 382200
References:
- Zheng et al (2019) Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 5 1602 PMID: 31572787
Products are for research use only. Not for human use.